3TNJ
Crystal structure of universal stress protein from Nitrosomonas europaea with AMP bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-15 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 77.809, 77.809, 39.949 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.1913 |
| Rwork | 0.189 |
| R-free | 0.24081 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pfs |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.535 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.051 | 0.575 |
| Number of reflections | 9070 | |
| <I/σ(I)> | 29 | 2 |
| Completeness [%] | 93.7 | 97.5 |
| Redundancy | 5.7 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M BisTris PH 7.0, 46% PEGP400, 6% Xylitol,10mM AMP, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
