3TN0
Structure of mouse Va14Vb8.2NKT TCR-mouse CD1d-a-C-Galactosylceramide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95453 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.171, 86.368, 236.403 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.770 - 3.200 |
| R-factor | 0.2286 |
| Rwork | 0.225 |
| R-free | 0.28870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3he6 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.005 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.272 | 1.080 |
| Number of reflections | 20725 | |
| <I/σ(I)> | 7.2 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.1 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 15-17% PEG 10K, 0.1M ammonium acetat, 0.1M BisTris pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
