3TMY
CHEY FROM THERMOTOGA MARITIMA (MN-III)
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 295 |
Detector technology | AREA DETECTOR |
Collection date | 1995-09-15 |
Detector | XUONG-HAMLIN MULTIWIRE |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 109.658, 64.719, 33.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
Rwork | 0.182 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tmy |
RMSD bond length | 0.016 |
RMSD bond angle | 2.440 |
Data reduction software | SDMS ((DCREDUCE)) |
Data scaling software | SDMS ((SCALE)) |
Phasing software | AMoRE |
Refinement software | TNT (5E) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.370 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.090 | 0.222 |
Number of reflections | 12436 | |
<I/σ(I)> | 6.8 | 2.5 |
Completeness [%] | 97.0 | 94 |
Redundancy | 3.7 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7 | 0.2M AMMONIUM SULFATE, 0.1M HEPES BUFFER (PH 7.0), 25% PEG 4000, 15MM MANGANESE CHLORIDE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | reservoir | magnesium chloride | 15 (mM) | |
3 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
4 | 1 | reservoir | HEPES | 0.1 (M) | |
5 | 1 | reservoir | PEG4000 | 25 (%) |