3TMX

X-Ray Radiation Damage to HEWL Crystals soaked in 100mM Sodium Nitrate (Dose=1.9MGy)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	1.241
Spacegroup name	P 43 21 2
Unit cell lengths	79.678, 79.678, 37.655
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.040 - 1.900
R-factor	0.15576
Rwork	0.153
R-free	0.20974
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.028
RMSD bond angle	1.856
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	BUSTER
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall
Low resolution limit [Å]	56.340
High resolution limit [Å]	1.900
Number of reflections	10046
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP