3TMR

IrisFP, planar chromophore

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-11
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9330
Spacegroup name	P 21 21 21
Unit cell lengths	84.840, 96.510, 140.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.984 - 2.000
R-factor	0.1585
Rwork	0.157
R-free	0.19490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.224
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall
Low resolution limit [Å]	79.500
High resolution limit [Å]	2.000
Number of reflections	74096

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.4	293	Ammonium sulfate, bicine, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K