3TMD
Bd1817, a HDG"Y"P protein from Bdellovibrio bacteriovorus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 101.010, 101.010, 94.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.739 - 2.641 |
| R-factor | 0.2139 |
| Rwork | 0.212 |
| R-free | 0.25120 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.092 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.200 | 2.780 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.079 | |
| Number of reflections | 16481 | |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 97.2 | 97.5 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 2.1M DL-Malic Acid pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
