3TMB
Bd1817, a HDG"Y"P protein from Bdellovibrio bacteriovorus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-01 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.168, 45.451, 98.005 |
| Unit cell angles | 90.00, 113.29, 90.00 |
Refinement procedure
| Resolution | 37.292 - 1.700 |
| R-factor | 0.1949 |
| Rwork | 0.194 |
| R-free | 0.21940 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.995 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.300 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.053 | |
| Number of reflections | 72760 | |
| <I/σ(I)> | 25 | |
| Completeness [%] | 97.7 | 85.4 |
| Redundancy | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.1M Bis Tris pH 5.5, 0.2M Ammonium acetate, 25% w/v PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
