3TM9
Y29A mutant of Vitreoscilla stercoraria hemoglobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-08-12 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 85.194, 46.426, 62.873 |
| Unit cell angles | 90.00, 128.84, 90.00 |
Refinement procedure
| Resolution | 31.123 - 1.720 |
| R-factor | 0.1756 |
| Rwork | 0.172 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.481 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 32.500 | 32.500 | 1.864 |
| High resolution limit [Å] | 1.800 | 3.878 | 1.800 |
| Rmerge | 0.071 | 0.031 | 0.454 |
| Number of reflections | 20497 | ||
| <I/σ(I)> | 11.002 | 29.44 | 1.69 |
| Completeness [%] | 88.3 | 98 | 47.8 |
| Redundancy | 3.3 | 3.58 | 1.67 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | 27 mg/mL protein in 1.3M (NH4)2SO4, 0.05M NaH2P2O7, 3% ethylene glycol. Not streak-seeded, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
