3TM8
Bd1817, a HDG"Y"P protein from Bdellovibrio bacteriovorus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.170, 79.840, 84.750 |
| Unit cell angles | 90.00, 95.99, 90.00 |
Refinement procedure
| Resolution | 30.402 - 1.280 |
| R-factor | 0.1668 |
| Rwork | 0.165 |
| R-free | 0.19590 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.023 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.600 | 1.350 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Number of reflections | 161271 | |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 99.6 | 98.6 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.1M Bis Tris pH 5.5, 0.2M Ammonium Acetate, 25% w/v PEG 3350, 20% v/v DMSO, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
