3TM7
Processed Aspartate Decarboxylase Mutant with Asn72 mutated to Ala
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-07 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.9781 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 71.083, 71.083, 215.807 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.16142 |
| Rwork | 0.160 |
| R-free | 0.18409 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.613 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.043 | 0.288 |
| Number of reflections | 36655 | |
| <I/σ(I)> | 59.3 | 9.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.75 | 11.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 1.6-2.4M ammonium sulphate, 0.1M citric acid, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
