3TM3
Wild-type hemoglobin from Vitreoscilla stercoraria
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-03-06 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 75.156, 99.013, 41.015 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.932 - 1.750 |
| R-factor | 0.1737 |
| Rwork | 0.170 |
| R-free | 0.20410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.087 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.200 | 32.100 | 1.761 |
| High resolution limit [Å] | 1.700 | 3.663 | 1.700 |
| Rmerge | 0.055 | 0.026 | 0.529 |
| Number of reflections | 32704 | ||
| <I/σ(I)> | 10.21 | 31.7 | 1.237 |
| Completeness [%] | 98.8 | 94.7 | 100 |
| Redundancy | 3.05 | 2.87 | 3.04 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 25 mg/mL protein in 1.6M (NH4)2SO4, 0.05M NaH2P2O7, 3% ethylene glycol, streak-seeded, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
