3TLX
Crystal Structure of PF10_0086, adenylate kinase from plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | .97911 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.395, 76.663, 93.768 |
| Unit cell angles | 90.00, 100.78, 90.00 |
Refinement procedure
| Resolution | 34.560 - 2.750 |
| R-factor | 0.2272 |
| Rwork | 0.226 |
| R-free | 0.25960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.180 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.000 | 35.000 | 2.800 |
| High resolution limit [Å] | 2.750 | 7.440 | 2.750 |
| Rmerge | 0.074 | 0.039 | 0.635 |
| Number of reflections | 29685 | ||
| <I/σ(I)> | 9.4 | 2.175 | |
| Completeness [%] | 99.9 | 98.1 | 100 |
| Redundancy | 4.2 | 3.9 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 25% PEG 3350, 0.2 M Ammonium Acetate, 0.1 M Hepes, 5 mM ADP, 5 mM MgCl2, 0.3 mM glyglyglycine, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 7.5 |
