3TLW
The GLIC pentameric Ligand-Gated Ion Channel Loop2-21' oxidized mutant in a locally-closed conformation (LC2 subtype)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.600, 127.650, 159.810 |
| Unit cell angles | 90.00, 101.28, 90.00 |
Refinement procedure
| Resolution | 30.020 - 2.600 |
| R-factor | 0.2073 |
| Rwork | 0.206 |
| R-free | 0.22460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | BUSTER-TNT (BUSTER 2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.500 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.079 | 0.558 |
| Number of reflections | 107062 | |
| <I/σ(I)> | 11.6 | 11.6 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4 | 293 | pH 4, VAPOR DIFFUSION, temperature 293K |
