3TLU
The GLIC pentameric Ligand-Gated Ion Channel Loop2-24' oxidized mutant in a locally-closed conformation (LC1 subtype)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.330, 127.810, 159.090 |
| Unit cell angles | 90.00, 100.77, 90.00 |
Refinement procedure
| Resolution | 44.140 - 2.850 |
| R-factor | 0.2042 |
| Rwork | 0.203 |
| R-free | 0.22320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data scaling software | SCALA |
| Refinement software | BUSTER-TNT (BUSTER 2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.700 | 3.000 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.116 | 0.947 |
| Number of reflections | 79676 | |
| <I/σ(I)> | 10.9 | 2.1 |
| Completeness [%] | 98.0 | 97.7 |
| Redundancy | 5.8 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4 | 293 | pH 4, VAPOR DIFFUSION, temperature 293K |
