3TLT
The GLIC pentameric Ligand-Gated Ion Channel H11'F mutant in a locally-closed conformation (LC1 subtype)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.370, 129.020, 160.530 |
| Unit cell angles | 90.00, 102.14, 90.00 |
Refinement procedure
| Resolution | 27.040 - 3.300 |
| R-factor | 0.2248 |
| Rwork | 0.224 |
| R-free | 0.24260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.080 |
| Data scaling software | SCALA |
| Refinement software | BUSTER-TNT (BUSTER 2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.100 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.064 | 0.433 |
| Number of reflections | 49209 | |
| <I/σ(I)> | 11.7 | 2 |
| Completeness [%] | 91.1 | 92.8 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4 | 293 | pH 4, VAPOR DIFFUSION, temperature 293K |
