3TLS
The GLIC pentameric Ligand-Gated Ion Channel E19'P mutant in a locally-closed conformation (LC2 subtype)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9919 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.272, 131.074, 158.139 |
| Unit cell angles | 90.00, 101.92, 90.00 |
Refinement procedure
| Resolution | 44.210 - 3.200 |
| R-factor | 0.2046 |
| Rwork | 0.203 |
| R-free | 0.23020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data scaling software | SCALA |
| Refinement software | BUSTER-TNT (BUSTER 2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.210 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.121 | 0.750 |
| Number of reflections | 58339 | |
| <I/σ(I)> | 7.4 | 2 |
| Completeness [%] | 98.5 | 94.7 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4 | 293 | pH 4, VAPOR DIFFUSION, temperature 293K |
