3TLQ
Crystal structure of EAL-like domain protein YdiV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-31 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.576, 50.348, 111.072 |
| Unit cell angles | 90.00, 98.62, 90.00 |
Refinement procedure
| Resolution | 39.635 - 1.910 |
| R-factor | 0.1732 |
| Rwork | 0.172 |
| R-free | 0.20040 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.076 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.980 |
| High resolution limit [Å] | 1.910 | 4.110 | 1.910 |
| Rmerge | 0.081 | 0.053 | 0.197 |
| Number of reflections | 41951 | ||
| <I/σ(I)> | 17.53 | 25.53 | 5.48 |
| Completeness [%] | 99.9 | 99.6 | 94.1 |
| Redundancy | 3.5 | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | 2.0M Sodium/Potassium phosphate PH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
