3TLO
Crystal structure of HCoV-NL63 3C-like protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-01-18 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.144, 83.497, 64.852 |
| Unit cell angles | 90.00, 109.20, 90.00 |
Refinement procedure
| Resolution | 20.596 - 1.600 |
| R-factor | 0.1758 |
| Rwork | 0.175 |
| R-free | 0.20190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.032 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.600 | 20.600 | 1.690 |
| High resolution limit [Å] | 1.600 | 5.060 | 1.600 |
| Number of reflections | 82371 | ||
| Completeness [%] | 98.3 | 98 | 94.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 0.1M HEPES, 10% PEG 8000, 5% ethylene glycerol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
