3TLI
THERMOLYSIN (10% ISOPROPANOL SOAKED CRYSTALS)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 93.990, 93.990, 130.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.950 |
| Rwork | 0.166 |
| R-free | 0.21300 * |
| Structure solution method | ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.900 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.080 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.089 | 0.268 |
| Number of reflections | 24568 | |
| <I/σ(I)> | 5.9 | 2.8 |
| Completeness [%] | 97.0 | 95 |
| Redundancy | 5.7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 | pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | TLN | 4.6 (mM) | |
| 2 | 1 | 1 | DMSO | 45 (%) | |
| 3 | 1 | 1 | Tris-HCl | 50 (mM) | |
| 4 | 1 | 1 | 2.5 (M) |
