3TLE
Microcin C7 self immunity protein MccF in complex with glutamyl sulfamoyl adenylate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.359, 85.810, 73.006 |
| Unit cell angles | 90.00, 101.26, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.300 |
| R-factor | 0.17889 |
| Rwork | 0.178 |
| R-free | 0.19658 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tla |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.988 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.600 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 157419 | |
| <I/σ(I)> | 36.9 | 9.5 |
| Completeness [%] | 98.2 | 96.2 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 282 | 10% PEG 8000, 8% ethylene glycol, 0.1 M HEPES, pH 7.5, vapor diffusion, hanging drop, temperature 282K |
