3TLC
Microcin C7 self immunity protein MccF in complex with Microcin C7 antibiotic
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Detector technology | CCD |
Collection date | 2010-04-02 |
Detector | MARMOSAIC 300 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.317, 85.738, 72.962 |
Unit cell angles | 90.00, 101.34, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.300 |
R-factor | 0.1747 |
Rwork | 0.174 |
R-free | 0.18620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tla |
RMSD bond length | 0.005 |
RMSD bond angle | 1.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.5.0056) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.350 |
High resolution limit [Å] | 1.300 | 2.800 | 1.300 |
Rmerge | 0.050 | 0.029 | 0.282 |
Number of reflections | 156612 | ||
<I/σ(I)> | 10.9 | ||
Completeness [%] | 97.2 | 99.6 | 93.8 |
Redundancy | 4.4 | 4.6 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 282 | 10% PEG 8000, 8% ethylene glycol, 0.1 M HEPES, pH 7.5, vapor diffusion, hanging drop, temperature 282K |