3TLA
Microcin C7 self immunity protein MccF in the wild type APO state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.424, 85.916, 73.056 |
| Unit cell angles | 90.00, 101.17, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.201 |
| R-factor | 0.1845 |
| Rwork | 0.184 |
| R-free | 0.20090 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.969 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0056) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.240 |
| High resolution limit [Å] | 1.200 | 2.590 | 1.200 |
| Rmerge | 0.068 | 0.049 | 0.352 |
| Number of reflections | 202577 | ||
| <I/σ(I)> | 9.7 | ||
| Completeness [%] | 98.9 | 99.5 | 93.8 |
| Redundancy | 7.3 | 7.5 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 282 | 10% PEG 8000, 8% ethylene glycol, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 282K |
