3TL8
The AvrPtoB-BAK1 complex reveals two structurally similar kinaseinteracting domains in a single type III effector
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2009-07-05 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.836, 108.140, 83.247 |
| Unit cell angles | 90.00, 92.67, 90.00 |
Refinement procedure
| Resolution | 24.141 - 2.500 |
| R-factor | 0.1864 |
| Rwork | 0.184 |
| R-free | 0.23780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qkw |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.060 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: dev_596)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 55.000 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 63725 | |
| Completeness [%] | 86.0 | 87.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.6M NH4Ac, PEG 3350 (v/v), pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
