3TKY
Monolignol o-methyltransferase (momt)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.855, 152.160, 68.181 |
| Unit cell angles | 90.00, 94.92, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.470 |
| R-factor | 0.197 |
| Rwork | 0.193 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kyz |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.828 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.470 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.130 | 0.500 |
| Number of reflections | 52117 | |
| <I/σ(I)> | 14 | 3.5 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 5.6 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.1 | 295 | 27% PEG 4000, 0.3M MAGNESIUM NITRATE AND 2 MM DTT, PH 7.1, VAPOR DIFFUSION, TEMPERATURE 295K |
