3TKN

Structure of the Nup82-Nup159-Nup98 heterotrimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-07-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03320
Spacegroup name	P 1 21 1
Unit cell lengths	106.905, 115.849, 118.488
Unit cell angles	90.00, 111.06, 90.00

Refinement procedure

Resolution	20.000 - 3.400
Rwork	0.249
R-free	0.28530
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.520
High resolution limit [Å]	3.400	3.400
Rmerge		0.018
Number of reflections	34404
Completeness [%]	92.8	96.2
Redundancy		4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	18.5% PEG3350, 100 mM potassium thiocyanate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K