3TKF

1.5 Angstrom Resolution Crystal Structure of K135M Mutant of Transaldolase B (TalA) from Francisella tularensis in Complex with Sedoheptulose 7-phosphate.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 21 21 21
Unit cell lengths	54.827, 87.194, 141.136
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.670 - 1.500
R-factor	0.14459
Rwork	0.144
R-free	0.15866
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3te9
RMSD bond length	0.012
RMSD bond angle	1.517
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.069	0.452
Number of reflections	109948
<I/σ(I)>	23.1	3.7
Completeness [%]	100.0	100
Redundancy	7.2	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	Protein:9.4mG/mL, 0.5M Sodium chloride, 0.1M TRIS-HCl (pH 8.3), 0.015M Sedoheptulose 7-phosphate; Screen: PEG's (B6), 0.1M HEPES, 25% (w/v) PEG2000 MME., VAPOR DIFFUSION, SITTING DROP, temperature 295K