3TK4
Crystal structure of phenylalanine hydroxylase from Chromobacterium violaceum bound to cobalt
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-03-27 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.885, 38.577, 47.877 |
| Unit cell angles | 76.49, 73.15, 85.39 |
Refinement procedure
| Resolution | 37.510 - 1.500 |
| R-factor | 0.17275 |
| Rwork | 0.171 |
| R-free | 0.20965 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ltu |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.324 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.031 | |
| Number of reflections | 37372 | |
| <I/σ(I)> | 24.2 | |
| Completeness [%] | 95.1 | 82.5 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M Na-HEPES, 0.001M Magnesium chloride hexahydrate, 15% w/v PEG 3,350, 0.1M Hexammine cobalt (III) chloride, 1.0M Guanidine hydrochloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
