3TJ0
Crystal Structure of Influenza B Virus Nucleoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97941 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 106.951, 123.342, 198.083 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.055 - 3.233 |
| R-factor | 0.2474 |
| Rwork | 0.245 |
| R-free | 0.29140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q06 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.729 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.350 |
| High resolution limit [Å] | 3.200 | 3.230 |
| Rmerge | 0.387 | |
| Number of reflections | 19883 | |
| <I/σ(I)> | 2.9 | |
| Completeness [%] | 93.1 | 95.1 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 1.4M sodium-potassium phosphate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
