3TIX

Crystal structure of the Chp1-Tas3 complex core

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-31
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	C 2 2 21
Unit cell lengths	104.880, 172.200, 198.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.063 - 2.900
R-factor	0.2123
Rwork	0.211
R-free	0.24270
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.526
Data reduction software	XDS ((VERSION February 3)
Data scaling software	XDS ((VERSION February 3)
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.700	2.980
High resolution limit [Å]	2.900	2.900
Rmerge	0.069	0.480
Number of reflections	40143
<I/σ(I)>	8.04	1.78
Completeness [%]	99.7	99.8
Redundancy	1.93	1.93

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	290	microseeding in 0.95 M potassium sodium tartrate, 100 mM MES, 180 mM sodium thiocyanate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 290K