3TIW
Crystal structure of p97N in complex with the C-terminus of gp78
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 32 |
| Unit cell lengths | 70.240, 70.240, 74.820 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.120 - 1.802 |
| R-factor | 0.1482 |
| Rwork | 0.147 |
| R-free | 0.17050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s3s |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.057 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.15) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.2_432) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 60.829 | 35.120 | 1.900 |
| High resolution limit [Å] | 1.802 | 5.700 | 1.800 |
| Rmerge | 0.106 | 0.071 | 0.492 |
| Total number of observations | 4619 | 21054 | |
| Number of reflections | 38133 | ||
| <I/σ(I)> | 7.5 | 6.8 | 1.6 |
| Completeness [%] | 100.0 | 99.7 | 100 |
| Redundancy | 3.8 | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 18-22% (w/v) polyethylene glycol 3000, 0.2 M NaCl and 100 mM Tris pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
