3TIQ
Crystal structure of Staphylococcus aureus SasG G51-E-G52 module
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-23 |
| Detector | ADSC Q315 |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.486, 84.771, 71.689 |
| Unit cell angles | 90.00, 100.39, 90.00 |
Refinement procedure
| Resolution | 41.297 - 1.874 |
| R-factor | 0.2083 |
| Rwork | 0.205 |
| R-free | 0.26620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tip |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.434 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.990 |
| High resolution limit [Å] | 1.870 | 5.580 | 1.870 |
| Rmerge | 0.053 | 0.028 | 0.367 |
| Number of reflections | 43216 | 1689 | 6474 |
| <I/σ(I)> | 14.9 | 36.62 | 3.02 |
| Completeness [%] | 98.1 | 97.6 | 90.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.2M magnesium chloride, 0.1M Bis-Tris, 25% PEG3350, pH 5.5, vapor diffusion sitting drop, temperature 293K |
