3TIO
Crystal structures of yrdA from Escherichia coli, a homologous protein of gamma-class carbonic anhydrase, show possible allosteric conformations
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.976, 84.384, 97.758 |
Unit cell angles | 90.00, 93.18, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.410 |
R-factor | 0.1927 |
Rwork | 0.193 |
R-free | 0.20980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1v3w |
RMSD bond length | 0.004 |
RMSD bond angle | 1.356 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.430 |
High resolution limit [Å] | 1.410 | 1.410 |
Number of reflections | 173327 | |
Completeness [%] | 99.3 | 99.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 4.6 | 295 | 20 % (w/v) polyethyleneglycol Monomethyl Ether 2000, 0.15M (NH4)2SO4, 0.1M Sodium Acetate, pH 4.6, EVAPORATION, temperature 295K |