3TIN
Tubulin tyrosine ligase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.99990 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.970, 75.720, 44.230 |
| Unit cell angles | 90.00, 90.83, 90.00 |
Refinement procedure
| Resolution | 29.240 - 2.900 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.31300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 8396 | |
| <I/σ(I)> | 29 | 5 |
| Completeness [%] | 98.7 | 95.9 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 0.1M Hepes 7.0, 14% Peg8000, 5mM MgCl2, 1mM ADP, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
