3TIJ
Crystal structure of a concentrative nucleoside transporter from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.06223 |
| Spacegroup name | P 63 |
| Unit cell lengths | 119.717, 119.717, 83.136 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.755 - 2.436 |
| R-factor | 0.1975 |
| Rwork | 0.196 |
| R-free | 0.22780 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.937 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.460 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.057 | 0.588 |
| Number of reflections | 49037 | |
| <I/σ(I)> | 28.256 | 2.2 |
| Completeness [%] | 99.8 | 98.3 |
| Redundancy | 7.9 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch-under-oil | 9 | 290 | 100 mM CaCl2, 40% PEG400, 100 mM Tris pH 9.0, microbatch-under-oil, temperature 290K |
