3TII
Tubulin tyrosine ligase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.416, 74.663, 117.328 |
| Unit cell angles | 90.00, 90.11, 90.00 |
Refinement procedure
| Resolution | 29.380 - 2.500 |
| R-factor | 0.254 |
| Rwork | 0.254 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 26519 | |
| <I/σ(I)> | 37.3 | 4.5 |
| Completeness [%] | 99.1 | 98.5 |
| Redundancy | 7.4 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 11% Peg3350, 0.1M MES pH 6.0, 1mM AMPPNP, 5mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
