3TIG
Tubulin tyrosine ligase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Detector technology | CCD |
| Collection date | 2010-06-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0781, 0.9806, 0.9637 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.570, 76.220, 44.240 |
| Unit cell angles | 90.00, 90.64, 90.00 |
Refinement procedure
| Resolution | 44.240 - 2.500 |
| R-factor | 0.25 |
| Rwork | 0.250 |
| R-free | 0.29800 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 13383 | |
| <I/σ(I)> | 52 | 5 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 0.1M Hepes 7.5, 8% Peg8000, 8% ethylene glycol, 0.05M Magnesium Chloride, 10mM Barium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
