3THH
Crystal structure of the Co2+2-HAI-ABH complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 3 |
Unit cell lengths | 90.428, 90.428, 69.431 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.850 |
R-factor | 0.2393 |
Rwork | 0.239 |
R-free | 0.28130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2aeb |
RMSD bond length | 0.007 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.078 | 0.586 |
Number of reflections | 52538 | |
<I/σ(I)> | 19.548 | 2.324 |
Completeness [%] | 99.3 | 98.4 |
Redundancy | 4.6 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | An unliganded Co2+2-HAI crystal was soaked in 20 mM ABH, 5 mM CoCl2, 100 mM HEPES, 30% (v/v) Jeffamine ED-2001 for 39 hours, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |