3TGR
Crystal structure of unliganded HIV-1 clade C strain C1086 gp120 core
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-04 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 67.860, 127.510, 193.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.289 - 2.800 |
R-factor | 0.2259 |
Rwork | 0.223 |
R-free | 0.28190 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.607 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
Rmerge | 0.079 | 0.048 | 0.294 |
Number of reflections | 20001 | ||
<I/σ(I)> | 16.1 | ||
Completeness [%] | 93.8 | 85.8 | 82.4 |
Redundancy | 4.2 | 4.2 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 16 % PEG 1500, 0.1M CaCl2, 0.1M imidazole, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 6.5 |