3TGP
Room temperature H-ras
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-16 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 40.100, 40.100, 160.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.875 - 1.308 |
| R-factor | 0.1691 |
| Rwork | 0.166 |
| R-free | 0.21320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.724 |
| Refinement software | PHENIX ((phenix.refine: dev_835)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.000 | 1.340 |
| High resolution limit [Å] | 1.308 | 1.308 |
| Number of reflections | 31564 | |
| Completeness [%] | 87.4 | 47 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.6 | 291 | Protein was crystallized from 28 % PEG 400, 10mM MgCl 264 mM TRIS/HCl, PH 7.6, pH 7.60, micro-batch, temperature 291.0K |
