3TGL
STRUCTURE AND MOLECULAR MODEL REFINEMENT OF RHIZOMUCOR MIEHEI TRIACYLGLYCERIDE LIPASE: A CASE STUDY OF THE USE OF SIMULATED ANNEALING IN PARTIAL MODEL REFINEMENT
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.600, 75.000, 55.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.500 - 1.900 |
| R-factor | 0.129 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.079 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 3.450 * |
| High resolution limit [Å] | 1.900 * |
| Completeness [%] | 82.8 * |
| Redundancy | 5.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15-16 (mg/ml) | |
| 2 | 1 | drop | Tris/HCl | ||
| 3 | 1 | reservoir | phosphate | 55-75 (%(v/v)sat) |
