3TGH
GAP50 the anchor in the inner membrane complex of Plasmodium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 57.648, 57.648, 210.625 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.440 - 1.700 |
| R-factor | 0.17454 |
| Rwork | 0.173 |
| R-free | 0.19874 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1war |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.022 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.742 | |
| Number of reflections | 43472 | |
| <I/σ(I)> | 18.7 | 2.08 |
| Completeness [%] | 97.3 | 100 |
| Redundancy | 5.2 | 4.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6 | 295 | 0.2 M (NH4)2SO4, 0.1 M sodium acetate pH 6.0, 3% DMSO or 3 % ethanol, 40% PEG8000, EVAPORATION, temperature 295K |
