3TGC
Crystal structure of L130R mutant of Nitrophorin 4 from Rhodnius prolixus complexed with nitrite at pH 7.4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-15 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.91841 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.762, 43.105, 52.428 |
| Unit cell angles | 90.00, 94.40, 90.00 |
Refinement procedure
| Resolution | 30.590 - 1.400 |
| R-factor | 0.1513 |
| Rwork | 0.149 |
| R-free | 0.18700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.048 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.590 | 1.440 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.038 | 0.285 |
| Number of reflections | 30602 | |
| Completeness [%] | 99.6 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 297 | Ammonium phosphate, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
