3TG8

Mutant ribosomal protein L1 lacking ala158 from thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-10
Detector	RAYONIX MX-225
Wavelength(s)	0.91841
Spacegroup name	C 2 2 21
Unit cell lengths	63.628, 104.473, 64.287
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.144 - 1.950
R-factor	0.1864
Rwork	0.184
R-free	0.22620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ad2
RMSD bond length	0.007
RMSD bond angle	1.080
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.100
High resolution limit [Å]	1.950	1.950
Rmerge	0.099
Number of reflections	15656
<I/σ(I)>	11.94	2.45
Completeness [%]	97.9	97.6
Redundancy	3.94	3.48

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	30 mM Tris-HCl, 75 mM NaCl with of 15% PEG 4K, 50 mM Tris-HCl, 0.2 M KBr (# 10 of Clear Strategy Screen I (MDL)) and 14% 2-methyl-2,4-pentandiol, 0.25 M MgCl2 in a 4:1:1, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 7.5