3TG0
E. coli alkaline phosphatase with bound inorganic phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.963, 98.457, 152.146 |
| Unit cell angles | 90.00, 94.61, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.200 |
| R-factor | 0.14948 |
| Rwork | 0.148 |
| R-free | 0.16869 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1alk |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.420 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Number of reflections | 501190 | |
| <I/σ(I)> | 15.9 | 3.1 |
| Completeness [%] | 86.0 | 28.3 |
| Redundancy | 3.4 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 20% PEG 4000, 0.2 M HEPES, 1 mM ZnCl2, 0.01 mM MgCl2, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
