3TFE

Crystal structure of an H-NOX protein from Nostoc sp. PCC 7120, L66W mutant under 6 atm of xenon

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.40
Spacegroup name	P 21 3
Unit cell lengths	123.295, 123.295, 123.295
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.591 - 1.991
R-factor	0.1567
Rwork	0.156
R-free	0.17310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tfd
RMSD bond length	0.009
RMSD bond angle	1.227
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.020
High resolution limit [Å]	1.991	1.991
Number of reflections	79661
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	1.9-2.0 M malonic acid, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K