3TFA

Crystal structure of an H-NOX protein from Nostoc sp. PCC 7120 under 6 atm of xenon

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.40
Spacegroup name	P 21 3
Unit cell lengths	123.937, 123.937, 123.937
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.818 - 2.271
R-factor	0.164
Rwork	0.163
R-free	0.19020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.267
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.310
High resolution limit [Å]	2.270	2.270
Number of reflections	55405
Completeness [%]	99.5	92.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.8 M DL-malic acid (pH 7.0) with and without 100 mM BIS-TRIS propane (pH 7.0), VAPOR DIFFUSION, HANGING DROP, temperature 293K