3TF9
Crystal structure of an H-NOX protein from Nostoc sp. PCC 7120 under 1 atm of xenon
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.40 |
Spacegroup name | P 21 3 |
Unit cell lengths | 124.404, 124.404, 124.404 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.983 - 2.590 |
R-factor | 0.1719 |
Rwork | 0.170 |
R-free | 0.20550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tf8 |
RMSD bond length | 0.021 |
RMSD bond angle | 1.881 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.623 |
High resolution limit [Å] | 2.590 | 2.590 |
Number of reflections | 36721 | |
Completeness [%] | 99.5 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.8 M DL-malic acid (pH 7.0) with and without 100 mM BIS-TRIS propane (pH 7.0), VAPOR DIFFUSION, HANGING DROP, temperature 293K |