3TF8

Crystal structure of an H-NOX protein from Nostoc sp. PCC 7120

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.977
Spacegroup name	P 21 3
Unit cell lengths	123.696, 123.696, 123.696
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.116 - 2.130
R-factor	0.166
Rwork	0.164
R-free	0.20000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2o09
RMSD bond length	0.010
RMSD bond angle	1.233
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.170
High resolution limit [Å]	2.130	2.130
Number of reflections	33688
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	1.8 M DL-malic acid (pH 7.0) with and without 100 mM BIS-TRIS propane (pH 7.0), VAPOR DIFFUSION, SITTING DROP, temperature 293K