3TF7
42F3 QL9/H2-Ld complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-17 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 102.487, 109.793, 213.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.450 - 2.750 |
| R-factor | 0.238 |
| Rwork | 0.237 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oi9 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.969 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.049 | |
| Number of reflections | 61762 | |
| Completeness [%] | 97.1 | 89.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.25 | 298 | 7% (w/v) PEG 17500, 100mM Bicine pH 8.25. 30% Glycerol cryo addiditive, VAPOR DIFFUSION, temperature 298K |
