3TF0

Crystal structure of an H-NOX protein from T. tengcongensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.977
Spacegroup name	P 21 21 2
Unit cell lengths	80.502, 130.766, 42.736
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.470 - 1.743
R-factor	0.2034
Rwork	0.202
R-free	0.22940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u4h
RMSD bond length	0.008
RMSD bond angle	0.992
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.800
High resolution limit [Å]	1.743	1.743
Number of reflections	43735
Completeness [%]	93.1	90.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	20-28% PEG 2000, 200-250 mM Sodium Acetate (pH 7.5), VAPOR DIFFUSION, SITTING DROP, temperature 293K